Hotspot

FNM16_pgt002-rps16

LSC · 6471-7216 · 746 bp

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Pi 0.0255
MarkerSeek score 59.10
Primer available yes
Label FNM16_pgt002-rps16

Feature Metadata

label_name
FNM16_pgt002-rps16
feature_type
igs
region
LSC
length_bp
746
variable_sites
19
pi
0.0255
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
FNM16_pgt002-rps16_p1 TCAGGATCAGTCGTGGTCTT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.1 58.0 974-977 bp 1.0 81.4
FNM16_pgt002-rps16_p2 TTCAGGATCAGTCGTGGTCT TTTCCTTGAAAGGGGTGCTC 50.0 50.0 58.1 58.0 975-978 bp 1.0 81.4
FNM16_pgt002-rps16_p3 TCAGGATCAGTCGTGGTCTT CGTTGCAATTGATGTTCGCT 50.0 45.0 58.1 58.0 1082-1085 bp 1.0 81.0
FNM16_pgt002-rps16_p4 TTCAGGATCAGTCGTGGTCT CGTTGCAATTGATGTTCGCT 50.0 45.0 58.1 58.0 1083-1086 bp 1.0 81.0
FNM16_pgt002-rps16_p5 TCAGGATCAGTCGTGGTCTT TCGTTGCAATTGATGTTCGC 50.0 45.0 58.1 58.0 1083-1086 bp 1.0 80.9