Hotspot

trnH-GUG-psbA

LSC · 1457-2123 · 667 bp

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Pi 0.037
MarkerSeek score 72.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
667
variable_sites
24
pi
0.037
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCTGTTGCCGAAGTTTCATC 55.0 50.0 58.1 58.0 722-814 bp 1.0 83.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCTGTTGCCGAAGTTTCATC 50.0 50.0 57.9 58.0 736-828 bp 1.0 83.0
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCTGTTGCCGAAGTTTCATC 50.0 50.0 57.9 58.0 735-827 bp 1.0 83.0
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCTGTTGCCGAAGTTTCATC 50.0 50.0 57.9 58.0 737-829 bp 1.0 83.0
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG AGACTTAGCTGTTGCCGAAG 55.0 50.0 58.1 57.9 729-821 bp 1.0 82.6