Hotspot

rps19-trnH-GUG

LSC · 280-1380 · 1101 bp

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Pi 0.0338
MarkerSeek score 63.40
Primer available yes
Label rps19-trnH-GUG

Feature Metadata

label_name
rps19-trnH-GUG
feature_type
igs
region
LSC
length_bp
1101
variable_sites
29
pi
0.0338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.78
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-trnH-GUG_p1 GAAAGGAGCACGTACCGATT GTAGAGTTAGAGGGGCGGAT 50.0 55.0 58.0 58.0 1135-1309 bp 1.0 85.9
rps19-trnH-GUG_p2 GAAAGGAGCACGTACCGATT TAGAGTTAGAGGGGCGGATG 50.0 55.0 58.0 58.0 1134-1308 bp 1.0 85.9
rps19-trnH-GUG_p3 TAGTTACTTGGTCTCGGGCA GTAGAGTTAGAGGGGCGGAT 50.0 55.0 58.1 58.0 1204-1378 bp 1.0 85.3
rps19-trnH-GUG_p4 TAGTTACTTGGTCTCGGGCA TAGAGTTAGAGGGGCGGATG 50.0 55.0 58.1 58.0 1203-1377 bp 1.0 85.3
rps19-trnH-GUG_p5 AATGGAAAGGAGCACGTACC GTAGAGTTAGAGGGGCGGAT 50.0 55.0 57.9 58.0 1139-1313 bp 1.0 85.1