Hotspot

ndhG

SSC · 120980-121510 · 531 bp

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Pi 0.00753

MarkerSeek score 48.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 4
pi: 0.00753
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GAAACAATAGCCAGCCCGAT	GGACGTTTACCAATGTCGGT	50.0	50.0	58.3	58.2	1575-1579 bp	1.0	76.9
ndhG_p2	GAAACAATAGCCAGCCCGAT	TGGGACGTTTACCAATGTCG	50.0	50.0	58.3	58.2	1577-1581 bp	1.0	76.9
ndhG_p3	TGTTCGAGCATCATTGACCA	GGACGTTTACCAATGTCGGT	45.0	50.0	57.5	58.2	1344-1348 bp	1.0	75.8
ndhG_p4	TGTTCGAGCATCATTGACCA	TGGGACGTTTACCAATGTCG	45.0	50.0	57.5	58.2	1346-1350 bp	1.0	75.8
ndhG_p5	AAGAAACAATAGCCAGCCCG	GGACGTTTACCAATGTCGGT	50.0	50.0	58.5	58.2	1577-1581 bp	1.0	75.2