Hotspot

ndhG-ndhI

SSC · 121511-122027 · 517 bp

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Pi 0.0135

MarkerSeek score 64.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 517
variable_sites: 7
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACAATACAACTCCCAGACTTCC	GGACGTTTACCAATGTCGGT	45.5	50.0	57.7	58.2	662-668 bp	1.0	60.9
ndhG-ndhI_p2	ACAATACAACTCCCAGACTTCC	TGGGACGTTTACCAATGTCG	45.5	50.0	57.7	58.2	664-670 bp	1.0	60.9
ndhG-ndhI_p3	CAATACAACTCCCAGACTTCCT	GGACGTTTACCAATGTCGGT	45.5	50.0	57.4	58.2	661-667 bp	1.0	58.8
ndhG-ndhI_p4	CAATACAACTCCCAGACTTCCT	TGGGACGTTTACCAATGTCG	45.5	50.0	57.4	58.2	663-669 bp	1.0	58.7
ndhG-ndhI_p5	ACAACTCCCAGACTTCCTAA	GGACGTTTACCAATGTCGGT	45.0	50.0	55.4	58.2	657-663 bp	1.0	58.3