Hotspot

ndhD-psaC

SSC · 119770-119902 · 133 bp

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Pi 0.0301

MarkerSeek score 64.00

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 133
variable_sites: 4
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TAAGGAACCCCCGGCTATAG	AAACAACTCGAAGCATGGGT	55.0	45.0	58.0	58.0	225 bp	1.0	81.1
ndhD-psaC_p2	TAAGGAACCCCCGGCTATAG	GGCATGAAACAACTCGAAGC	55.0	50.0	58.0	58.0	231 bp	1.0	81.0
ndhD-psaC_p3	ATTAAGGAACCCCCGGCTAT	AAACAACTCGAAGCATGGGT	50.0	45.0	58.2	58.0	227 bp	1.0	79.9
ndhD-psaC_p4	ATTAAGGAACCCCCGGCTAT	GGCATGAAACAACTCGAAGC	50.0	50.0	58.2	58.0	233 bp	1.0	79.8
ndhD-psaC_p5	TAAGGAACCCCCGGCTATAG	CAGAGGACTGTGTTGGTTGT	55.0	50.0	58.0	57.7	306 bp	1.0	78.2