Hotspot

rps11-rpl36

LSC · 81894-82340 · 447 bp

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Pi 0.0248
MarkerSeek score 57.40
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
447
variable_sites
11
pi
0.0248
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 TTTCGGTATATGTTTTGCCA AGGGCGAATTATAGTAATTTGTGC 35.0 37.5 53.2 57.7 519-523 bp 1.0 42.3
rps11-rpl36_p2 TTTCGGTATATGTTTTGCCA GGGCGAATTATAGTAATTTGTGCT 35.0 37.5 53.2 57.7 518-522 bp 1.0 42.3
rps11-rpl36_p3 TTTTCGGTATATGTTTTGCCA AGGGCGAATTATAGTAATTTGTGC 33.3 37.5 54.1 57.7 520-524 bp 1.0 42.3
rps11-rpl36_p4 TTTTCGGTATATGTTTTGCCA GGGCGAATTATAGTAATTTGTGCT 33.3 37.5 54.1 57.7 519-523 bp 1.0 42.3
rps11-rpl36_p5 TTCGGTATATGTTTTGCCAT GGGCGAATTATAGTAATTTGTGCT 35.0 37.5 52.9 57.7 517-521 bp 1.0 42.3