Hotspot

rpl33-rps18

LSC · 70080-70337 · 258 bp

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Pi 0.0233
MarkerSeek score 65.90
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
258
variable_sites
6
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 TCTGTCCTTGTTGTTGCAAA TCAATTCGATCCCCCGATTG 40.0 50.0 56.0 57.7 394-400 bp 1.0 63.2
rpl33-rps18_p2 TTCTGTCCTTGTTGTTGCAA TCAATTCGATCCCCCGATTG 40.0 50.0 56.0 57.7 395-401 bp 1.0 63.2
rpl33-rps18_p3 TTCTGTCCTTGTTGTTGCAAA TCAATTCGATCCCCCGATTG 38.1 50.0 56.8 57.7 395-401 bp 1.0 61.3
rpl33-rps18_p4 ATTCTGTCCTTGTTGTTGCA TCAATTCGATCCCCCGATTG 40.0 50.0 55.8 57.7 396-402 bp 1.0 61.3
rpl33-rps18_p5 CGACACAATACACCTAGTCG TCAATTCGATCCCCCGATTG 50.0 50.0 55.7 57.7 431-437 bp 1.0 61.0