Hotspot

trnP-UGG-psaJ

LSC · 68516-69271 · 756 bp

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Pi 0.0112

MarkerSeek score 68.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 8
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TCTATCAAGAGACCCGCCAA	50.0	50.0	58.1	58.1	894-1119 bp	1.0	82.0
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TCTATCAAGAGACCCGCCAA	50.0	50.0	58.1	58.1	893-1118 bp	1.0	82.0
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TCTATCAAGAGACCCGCCAA	50.0	50.0	58.1	58.1	905-1130 bp	1.0	81.9
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TCTATCAAGAGACCCGCCAA	50.0	50.0	58.1	58.1	904-1129 bp	1.0	81.9
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TCTATCAAGAGACCCGCCAA	50.0	50.0	58.1	58.1	907-1132 bp	1.0	81.9