Hotspot

petA-psbJ

LSC · 65134-66025 · 892 bp

Back to Kotschya

Pi 0.0125

MarkerSeek score 67.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 11
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTGCAAGGTCT	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1038-1045 bp	1.0	75.6
petA-psbJ_p2	TCCATTACGTGTGCAAGGTC	CATATTCCGGATTGGGCTCA	50.0	50.0	58.2	57.4	1039-1046 bp	1.0	75.6
petA-psbJ_p3	CCATTACGTGTGCAAGGTCT	TATTCCGGATTGGGCTCATC	50.0	50.0	58.2	57.1	1036-1043 bp	1.0	73.3
petA-psbJ_p4	CCATTACGTGTGCAAGGTCT	TCATATTCCGGATTGGGCTC	50.0	50.0	58.2	57.1	1039-1046 bp	1.0	73.3
petA-psbJ_p5	TCCATTACGTGTGCAAGGTC	TATTCCGGATTGGGCTCATC	50.0	50.0	58.2	57.1	1037-1044 bp	1.0	73.2