Hotspot

trnG-UCC-trnS-GCU

LSC · 55513-56092 · 580 bp

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Pi 0.0261
MarkerSeek score 74.10
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
580
variable_sites
15
pi
0.0261
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 GGGGTCTGTAGAACGAATCG CAATCCGACGCTTTAGTCCA 55.0 50.0 57.8 58.0 657-661 bp 1.0 80.7
trnG-UCC-trnS-GCU_p2 GGGGTCTGTAGAACGAATCG AATCCGACGCTTTAGTCCAC 55.0 50.0 57.8 58.0 656-660 bp 1.0 80.7
trnG-UCC-trnS-GCU_p3 GGTCTGTAGAACGAATCGCA CAATCCGACGCTTTAGTCCA 50.0 50.0 57.7 58.0 655-659 bp 1.0 80.2
trnG-UCC-trnS-GCU_p4 GGTCTGTAGAACGAATCGCA AATCCGACGCTTTAGTCCAC 50.0 50.0 57.7 58.0 654-658 bp 1.0 80.2
trnG-UCC-trnS-GCU_p5 GGGGTCTGTAGAACGAATCG ACGGAAAGAGAGGGATTCGA 55.0 50.0 57.8 58.2 710-714 bp 1.0 79.4