Hotspot

atpH-atpF

LSC · 50679-51166 · 488 bp

Back to Kotschya

Pi 0.0166

MarkerSeek score 66.90

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 488
variable_sites: 8
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	AGCAGAGGGAAAAATACGGG	CGGATGGCCAATAACCCAAA	50.0	50.0	57.6	58.5	652-655 bp	1.0	74.1
atpH-atpF_p2	GCAGAGGGAAAAATACGGGG	CGGATGGCCAATAACCCAAA	55.0	50.0	58.6	58.5	651-654 bp	1.0	72.5
atpH-atpF_p3	AGCAGAGGGAAAAATACGGG	TTAAACCCGAAACTTCCGGC	50.0	50.0	57.6	58.8	671-674 bp	1.0	72.1
atpH-atpF_p4	CAGAGGGAAAAATACGGGGT	CGGATGGCCAATAACCCAAA	50.0	50.0	57.2	58.5	650-653 bp	1.0	71.0
atpH-atpF_p5	GCAGAGGGAAAAATACGGGG	TTAAACCCGAAACTTCCGGC	55.0	50.0	58.6	58.8	670-673 bp	1.0	70.6