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psbM-petN

LSC · 33589-34583 · 995 bp

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Pi 0.0154

MarkerSeek score 69.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 15
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	TCTTGCTTGGGCTGCTTTAA	42.9	45.0	57.6	58.0	1129-1150 bp	1.0	71.3
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	AGTAAGTCTTGCTTGGGCTG	42.9	50.0	57.6	57.8	1135-1156 bp	1.0	69.8
psbM-petN_p3	TGCTACTGCACTGTTCATTC	TCTTGCTTGGGCTGCTTTAA	45.0	45.0	56.1	58.0	1129-1150 bp	1.0	67.1
psbM-petN_p4	TGCTACTGCACTGTTCATTC	AGTAAGTCTTGCTTGGGCTG	45.0	50.0	56.1	57.8	1135-1156 bp	1.0	65.6
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	TTGCTTGGGCTGCTTTAATG	42.9	45.0	57.6	57.3	1127-1148 bp	1.0	65.4