Hotspot

trnG-GCC-psbZ

LSC · 25898-26736 · 839 bp

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Pi 0.0159

MarkerSeek score 61.90

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 13
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGATGGTTGGTCG	50.0	55.0	57.8	58.0	993-1043 bp	1.0	81.0
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	TTGCTTCTCCTGATGGTTGG	50.0	50.0	57.8	57.8	996-1046 bp	1.0	79.5
trnG-GCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	TCCTGATGGTTGGTCGAGTA	50.0	50.0	57.8	57.8	989-1039 bp	1.0	79.4
trnG-GCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	GCTTCTCCTGATGGTTGGTC	50.0	55.0	57.8	58.3	994-1044 bp	1.0	79.1
trnG-GCC-psbZ_p5	AAGGGAGGAGGAATACAGGC	CTTCTCCTGATGGTTGGTCG	55.0	55.0	58.5	58.0	1034-1084 bp	1.0	78.4