Hotspot

trnF-GAA-trnL-UAA

LSC · 13449-14000 · 552 bp

Back to Kotschya

Pi 0.0173

MarkerSeek score 61.70

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 552
variable_sites: 9
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TCTACCAGCTGAGCTATCCC	AATCGTGAGGGTTCAAGTCC	55.0	50.0	58.0	57.5	576-606 bp	1.0	81.4
trnF-GAA-trnL-UAA_p2	TTTGAACTGGTGACACGAGG	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	612-642 bp	1.0	80.9
trnF-GAA-trnL-UAA_p3	GTGCCAGGAACCAGATTTGA	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	627-657 bp	1.0	80.5
trnF-GAA-trnL-UAA_p4	TCAGTCCTCTGCTCTACCAG	AATCGTGAGGGTTCAAGTCC	55.0	50.0	57.9	57.5	588-618 bp	1.0	80.3
trnF-GAA-trnL-UAA_p5	CATGTGCCAGGAACCAGATT	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.2	57.5	630-660 bp	1.0	79.9