Hotspot

matK-trnK-UUU

LSC · 3825-4637 · 813 bp

Back to Kotschya

Pi 0.00861

MarkerSeek score 63.70

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 813
variable_sites: 7
pi: 0.00861
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	GGAAGTTCTGTTGGCGAGAA	CGGGACAAAGGGATGTTCAT	50.0	50.0	58.1	57.9	980-988 bp	1.0	78.6
matK-trnK-UUU_p2	GGAAGTTCTGTTGGCGAGAA	GGGGTTGCTAACTCAATGGT	50.0	50.0	58.1	57.8	906-914 bp	1.0	77.9
matK-trnK-UUU_p3	ATGACCATAGGCGAGTCCAT	CGGGACAAAGGGATGTTCAT	50.0	50.0	58.3	57.9	1030-1038 bp	1.0	77.3
matK-trnK-UUU_p4	ATGACCATAGGCGAGTCCAT	GGGGTTGCTAACTCAATGGT	50.0	50.0	58.3	57.8	956-964 bp	1.0	76.6
matK-trnK-UUU_p5	TGACCATAGGCGAGTCCATA	CGGGACAAAGGGATGTTCAT	50.0	50.0	57.6	57.9	1029-1037 bp	1.0	76.5