Hotspot

psbA-trnK-UUU

LSC · 1411-1982 · 572 bp

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Pi 0.019

MarkerSeek score 60.00

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 572
variable_sites: 10
pi: 0.019
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	TCGCGTCTCTCTAAAATTGCA	CACGGTTTGGGGAGAGATTT	42.9	50.0	58.0	57.8	656-701 bp	1.0	75.2
psbA-trnK-UUU_p2	TCGCGTCTCTCTAAAATTGCA	AAAATGCAAGCACGGTTTGG	42.9	45.0	58.0	58.4	666-711 bp	1.0	73.4
psbA-trnK-UUU_p3	CGCGTCTCTCTAAAATTGCAG	CACGGTTTGGGGAGAGATTT	47.6	50.0	57.7	57.8	655-700 bp	1.0	73.2
psbA-trnK-UUU_p4	TCGCGTCTCTCTAAAATTGCA	TGAAAAATGCAAGCACGGTT	42.9	40.0	58.0	57.4	669-714 bp	1.0	72.0
psbA-trnK-UUU_p5	CGCGTCTCTCTAAAATTGCA	CACGGTTTGGGGAGAGATTT	45.0	50.0	56.6	57.8	655-700 bp	1.0	72.0