Hotspot

trnH-GUG-psbA

LSC · 120-348 · 229 bp

Back to Kotschya

Pi 0.0349

MarkerSeek score 66.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 8
pi: 0.0349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CAGTCGAGGCTCCTTCTCTA	55.0	55.0	58.1	58.0	279 bp	1.0	80.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CAGTCGAGGCTCCTTCTCTA	50.0	55.0	57.9	58.0	293 bp	1.0	80.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CAGTCGAGGCTCCTTCTCTA	50.0	55.0	57.9	58.0	292 bp	1.0	80.7
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CAGTCGAGGCTCCTTCTCTA	50.0	55.0	57.9	58.0	294 bp	1.0	80.7
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCAGT	55.0	55.0	58.1	57.9	295 bp	1.0	80.6