Hotspot

ycf1

SSC · 132656-138196 · 5541 bp

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Pi 0.0131
MarkerSeek score 48.40
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5541
variable_sites
108
pi
0.0131
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AAAATGGGGTCCAATGGCAT ACAGGAACAAGAGGGATCCA 45.0 50.0 58.0 58.0 1003 bp 1.0 82.3
ycf1_p2 AAATGGGGTCCAATGGCATT ACAGGAACAAGAGGGATCCA 45.0 50.0 58.0 58.0 1002 bp 1.0 82.3
ycf1_p3 AAATGGGGTCCAATGGCATT AAGAGGGATCCACCGAAGAA 45.0 50.0 58.0 58.0 994 bp 1.0 82.3
ycf1_p4 AAAATGGGGTCCAATGGCAT GCAGAATACCGTCACCCATT 45.0 50.0 58.0 58.0 1127 bp 1.0 82.1
ycf1_p5 AAATGGGGTCCAATGGCATT GCAGAATACCGTCACCCATT 45.0 50.0 58.0 58.0 1126 bp 1.0 82.1