Hotspot

trnN-GUU-trnR-ACG

IRa · 138597-139145 · 549 bp

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Pi 0.0085
MarkerSeek score 63.10
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
549
variable_sites
7
pi
0.0085
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 690 bp 1.0 79.1
trnN-GUU-trnR-ACG_p2 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 641 bp 1.0 77.9
trnN-GUU-trnR-ACG_p3 CGATTGGTCGTAGGTTCGAA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.7 57.8 645 bp 1.0 77.0
trnN-GUU-trnR-ACG_p4 TCTCCCGTTGTTCCTCAGTA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.7 57.8 694 bp 1.0 76.7
trnN-GUU-trnR-ACG_p5 TTCCTCAGTAGCTCAGTGGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.7 57.8 684 bp 1.0 76.6