Hotspot

ndhF-rpl32

SSC · 121903-122570 · 668 bp

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Pi 0.0216
MarkerSeek score 67.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
668
variable_sites
21
pi
0.0216
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAACGGGAAGTGGAATGAA GGATTCCTTATTGAATGTGAAAGACA 50.0 34.6 57.8 57.9 730-780 bp 1.0 46.2
ndhF-rpl32_p2 ACGGGAAGTGGAATGAAAGG GGATTCCTTATTGAATGTGAAAGACA 50.0 34.6 57.8 57.9 727-777 bp 1.0 46.2
ndhF-rpl32_p3 CGGGAAGTGGAATGAAAGGT GGATTCCTTATTGAATGTGAAAGACA 50.0 34.6 57.8 57.9 726-776 bp 1.0 46.2
ndhF-rpl32_p4 GGAACGGGAAGTGGAATGAA AGGATTCCTTATTGAATGTGAAAGAC 50.0 34.6 57.8 57.7 731-781 bp 1.0 46.2
ndhF-rpl32_p5 CGGGAAGTGGAATGAAAGGT AGGATTCCTTATTGAATGTGAAAGAC 50.0 34.6 57.8 57.7 727-777 bp 1.0 46.2