Hotspot

trnR-ACG-trnN-GUU

IRb · 117642-118190 · 549 bp

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Pi 0.0085
MarkerSeek score 63.10
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
549
variable_sites
7
pi
0.0085
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 690 bp 1.0 79.1
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 641 bp 1.0 77.9
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG CGATTGGTCGTAGGTTCGAA 55.0 50.0 57.8 57.7 645 bp 1.0 77.0
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG TCTCCCGTTGTTCCTCAGTA 55.0 50.0 57.8 57.7 694 bp 1.0 76.7
trnR-ACG-trnN-GUU_p5 CAGAGGATTAGAGCACGTGG TTCCTCAGTAGCTCAGTGGT 55.0 50.0 57.8 57.7 684 bp 1.0 76.6