Hotspot

ycf3-trnS-GGA

LSC · 54166-54711 · 546 bp

Back to Kopsia

Pi 0.011
MarkerSeek score 64.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
546
variable_sites
9
pi
0.011
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CTCCCTTTCTCCCGAAGTTG CCCTCGGTAAACAAAAGCCT 55.0 50.0 57.9 58.1 714-715 bp 1.0 79.6
ycf3-trnS-GGA_p2 CTCCCTTTCTCCCGAAGTTG ACGGAAAGAGAGGGATTCGA 55.0 50.0 57.9 58.2 735-736 bp 1.0 79.1
ycf3-trnS-GGA_p3 CCCTTTCTCCCGAAGTTGTC CCCTCGGTAAACAAAAGCCT 55.0 50.0 58.2 58.1 712-713 bp 1.0 78.8
ycf3-trnS-GGA_p4 CTCCCTTTCTCCCGAAGTTG TTCCAATGCTACGCCTTGAA 55.0 45.0 57.9 57.8 685-686 bp 1.0 78.8
ycf3-trnS-GGA_p5 CCCTTTCTCCCGAAGTTGTC ACGGAAAGAGAGGGATTCGA 55.0 50.0 58.2 58.2 733-734 bp 1.0 78.4