Hotspot

psbM-trnD-GUC

LSC · 39806-40351 · 546 bp

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Pi 0.0208
MarkerSeek score 66.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
546
variable_sites
17
pi
0.0208
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGCGGTAGGAACTAGAATGA TTCAATTGGTCAGAGCACCG 47.6 50.0 58.0 58.5 667-673 bp 1.0 70.5
psbM-trnD-GUC_p2 AGAAAGGCGGTAGGAACTAGA TTCAATTGGTCAGAGCACCG 47.6 50.0 57.9 58.5 671-677 bp 1.0 69.9
psbM-trnD-GUC_p3 AGTAGAAAGGCGGTAGGAACT TTCAATTGGTCAGAGCACCG 47.6 50.0 58.2 58.5 674-680 bp 1.0 69.2
psbM-trnD-GUC_p4 AGTAGAAAGGCGGTAGGAAC TTCAATTGGTCAGAGCACCG 50.0 50.0 56.7 58.5 674-680 bp 1.0 67.9
psbM-trnD-GUC_p5 GTAGAAAGGCGGTAGGAACT TTCAATTGGTCAGAGCACCG 50.0 50.0 56.7 58.5 673-679 bp 1.0 67.9