Hotspot

psbK-psbI

LSC · 7873-8027 · 155 bp

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Pi 0.0194
MarkerSeek score 63.30
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
155
variable_sites
3
pi
0.0194
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GCCTGAGGCCTATGCTTTTT AAACCCCTATTCTTCACGCC 50.0 50.0 58.5 57.9 376-381 bp 1.0 79.5
psbK-psbI_p2 CTTTTGTTTGGCAAGCTGCT AAGGGGTTTTTGTTTTCGCG 45.0 45.0 58.1 58.1 869-876 bp 0.666667 67.8
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG AAGGGGTTTTTGTTTTCGCG 50.0 45.0 57.9 58.1 864-871 bp 0.666667 67.8
psbK-psbI_p4 CTTTTGTTTGGCAAGCTGCT TCGAAGCGTTACGACAGAAG 45.0 50.0 58.1 58.0 886 bp 0.333333 52.6
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG TCGAAGCGTTACGACAGAAG 50.0 50.0 57.9 58.0 881 bp 0.333333 52.6