primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	77	348	290-621	1.000000	86.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.208010	77	348	304-635	1.000000	86.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	77	348	303-634	1.000000	86.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.209518	77	348	305-636	1.000000	86.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	20	20	55.0	55.0	58.1	58.3	0.0	0.0	0.465225	77	348	339-670	1.000000	83.7
trnK-UUU_p1	trnK-UUU	1	TGCTAGTGTCAACCAATATCGA	TGGGGAGGGGTGTTTACTTA	22	20	40.9	50.0	57.3	57.6	0.0	0.0	3.166191	1628	1662	189-189	1.000000	55.5
trnK-UUU_p2	trnK-UUU	2	TGCTAGTGTCAACCAATATCGA	TACATAGGGAAAGCCGTGTG	22	20	40.9	50.0	57.3	57.3	0.0	0.0	3.413228	1628	1662	232-232	1.000000	53.4
trnK-UUU_p3	trnK-UUU	3	TGCTAGTGTCAACCAATATCG	TGGGGAGGGGTGTTTACTTA	21	20	42.9	50.0	55.8	57.6	0.0	0.0	3.639370	1628	1662	189-189	1.000000	51.7
trnK-UUU_p4	trnK-UUU	4	TGCTAGTGTCAACCAATATCGA	CTGGGGAGGGGTGTTTACTT	22	20	40.9	55.0	57.3	58.9	0.0	0.0	3.656129	1628	1662	190-190	1.000000	51.6
trnK-UUU_p5	trnK-UUU	5	TGCTAGTGTCAACCAATATCGAT	TGGGGAGGGGTGTTTACTTA	23	20	39.1	50.0	57.7	57.6	2.2	0.0	3.713425	1628	1662	189-189	1.000000	51.1
psbK-psbI_p1	psbK-psbI	1	GCCTGAGGCCTATGCTTTTT	AAACCCCTATTCTTCACGCC	20	20	50.0	50.0	58.5	57.9	13.8	0.0	0.645647	7873	8027	376-381	1.000000	79.5
psbK-psbI_p2	psbK-psbI	2	CTTTTGTTTGGCAAGCTGCT	AAGGGGTTTTTGTTTTCGCG	20	20	45.0	45.0	58.1	58.1	15.2	0.0	0.126738	7873	8027	869-876	0.666667	67.8
psbK-psbI_p3	psbK-psbI	3	GTTTGGCAAGCTGCTGTAAG	AAGGGGTTTTTGTTTTCGCG	20	20	50.0	45.0	57.9	58.1	1.4	0.0	0.126864	7873	8027	864-871	0.666667	67.8
psbK-psbI_p4	psbK-psbI	4	CTTTTGTTTGGCAAGCTGCT	TCGAAGCGTTACGACAGAAG	20	20	45.0	50.0	58.1	58.0	15.2	9.6	0.096222	7873	8027	886-886	0.333333	52.6
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	TCGAAGCGTTACGACAGAAG	20	20	50.0	50.0	57.9	58.0	1.4	9.6	0.096347	7873	8027	881-881	0.333333	52.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	GCGATCGCATCTCGTTAGAT	TTGTTTTGCTCGGTTCTCGA	20	20	50.0	45.0	58.0	58.1	33.9	0.0	0.086876	12705	13704	1738-1738	0.333333	52.6
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	TTTCTCATCTCATCAGCCGC	TTGTTTTGCTCGGTTCTCGA	20	20	50.0	45.0	58.1	58.1	0.0	0.0	0.111935	12705	13704	2137-2137	0.333333	52.4
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AATGTTTCGTTCCGTCGGAT	TTGTTTTGCTCGGTTCTCGA	20	20	45.0	45.0	57.9	58.1	16.2	0.0	0.146272	12705	13704	1648-1648	0.333333	52.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TGGTCATTCCAAAGAGAGCG	TTGTTTTGCTCGGTTCTCGA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.156464	12705	13704	2168-2168	0.333333	52.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	GGTCATTCCAAAGAGAGCGT	TTGTTTTGCTCGGTTCTCGA	20	20	50.0	45.0	57.9	58.1	0.0	0.0	0.157927	12705	13704	2167-2167	0.333333	52.1
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.0	58.5	0.0	5.4	1.505426	39806	40351	667-673	1.000000	70.5
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAAAGGCGGTAGGAACTAGA	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	57.9	58.5	0.0	5.4	1.574673	39806	40351	671-677	1.000000	69.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AGTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	21	20	47.6	50.0	58.2	58.5	0.0	5.4	1.659667	39806	40351	674-680	1.000000	69.2
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGTAGAAAGGCGGTAGGAAC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.7	58.5	0.0	5.4	1.821422	39806	40351	674-680	1.000000	67.9
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	GTAGAAAGGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	56.7	58.5	0.0	5.4	1.821422	39806	40351	673-679	1.000000	67.9
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CTCCCTTTCTCCCGAAGTTG	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.194967	54166	54711	714-715	1.000000	79.6
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CTCCCTTTCTCCCGAAGTTG	ACGGAAAGAGAGGGATTCGA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.251953	54166	54711	735-736	1.000000	79.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCCTTTCTCCCGAAGTTGTC	CCCTCGGTAAACAAAAGCCT	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.284679	54166	54711	712-713	1.000000	78.8
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CTCCCTTTCTCCCGAAGTTG	TTCCAATGCTACGCCTTGAA	20	20	55.0	45.0	57.9	57.8	0.0	5.6	0.293268	54166	54711	685-686	1.000000	78.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CCCTTTCTCCCGAAGTTGTC	ACGGAAAGAGAGGGATTCGA	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.341664	54166	54711	733-734	1.000000	78.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAAGCCA	20	20	50.0	50.0	57.9	58.0	6.4	4.8	0.061479	74854	75902	1241-1382	1.000000	80.4
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAAGCCA	20	20	50.0	50.0	57.8	58.0	24.9	4.8	0.166488	74854	75902	1164-1305	1.000000	79.7
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAAGCCA	20	20	45.0	50.0	58.2	58.0	0.0	4.8	0.181166	74854	75902	1250-1391	1.000000	79.5
psbE-petL_p4	psbE-petL	4	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAAGCCA	20	20	50.0	50.0	58.2	58.0	0.0	4.8	0.194071	74854	75902	1255-1396	1.000000	79.4
psbE-petL_p5	psbE-petL	5	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAAGCCA	20	20	50.0	50.0	57.8	58.0	0.0	4.8	0.197496	74854	75902	1249-1390	1.000000	79.3
trnI-GAU_p1	trnI-GAU	1	AGAGAGGGATGGGGTTTCTC	TCTATTCTTTTCCCTGGCGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.151965	111784	112803	1105-1105	1.000000	79.1
trnI-GAU_p2	trnI-GAU	2	AGAGGGATGGGGTTTCTCTC	TCTATTCTTTTCCCTGGCGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.151965	111784	112803	1103-1103	1.000000	79.1
trnI-GAU_p3	trnI-GAU	3	GAGAGGGATGGGGTTTCTCT	TCTATTCTTTTCCCTGGCGC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.151965	111784	112803	1104-1104	1.000000	79.1
trnI-GAU_p4	trnI-GAU	4	GATGGGGTTTCTCTCGCTTT	TCTATTCTTTTCCCTGGCGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.153730	111784	112803	1098-1098	1.000000	79.1
trnI-GAU_p5	trnI-GAU	5	AGAGGGATGGGGTTTCTCTC	CGGAGCATGCATGAAGTAGT	20	20	55.0	50.0	58.1	58.1	0.0	30.6	0.168190	111784	112803	1134-1134	1.000000	79.0
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	117642	118190	690-690	1.000000	79.1
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	117642	118190	641-641	1.000000	77.9
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.483095	117642	118190	645-645	1.000000	77.0
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	TCTCCCGTTGTTCCTCAGTA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.513605	117642	118190	694-694	1.000000	76.7
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	TTCCTCAGTAGCTCAGTGGT	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.526686	117642	118190	684-684	1.000000	76.6
ndhF-rpl32_p1	ndhF-rpl32	1	GGAACGGGAAGTGGAATGAA	GGATTCCTTATTGAATGTGAAAGACA	20	26	50.0	34.6	57.8	57.9	0.0	0.0	6.249480	121903	122570	730-780	1.000000	46.2
ndhF-rpl32_p2	ndhF-rpl32	2	ACGGGAAGTGGAATGAAAGG	GGATTCCTTATTGAATGTGAAAGACA	20	26	50.0	34.6	57.8	57.9	0.0	0.0	6.255651	121903	122570	727-777	1.000000	46.2
ndhF-rpl32_p3	ndhF-rpl32	3	CGGGAAGTGGAATGAAAGGT	GGATTCCTTATTGAATGTGAAAGACA	20	26	50.0	34.6	57.8	57.9	0.0	0.0	6.255651	121903	122570	726-776	1.000000	46.2
ndhF-rpl32_p4	ndhF-rpl32	4	GGAACGGGAAGTGGAATGAA	AGGATTCCTTATTGAATGTGAAAGAC	20	26	50.0	34.6	57.8	57.7	0.0	0.0	6.480393	121903	122570	731-781	1.000000	46.2
ndhF-rpl32_p5	ndhF-rpl32	5	CGGGAAGTGGAATGAAAGGT	AGGATTCCTTATTGAATGTGAAAGAC	20	26	50.0	34.6	57.8	57.7	0.0	0.0	6.486564	121903	122570	727-777	1.000000	46.2
ycf1_p1	ycf1	1	AAAATGGGGTCCAATGGCAT	ACAGGAACAAGAGGGATCCA	20	20	45.0	50.0	58.0	58.0	0.0	15.2	0.075080	137072	137191	1003-1003	1.000000	82.3
ycf1_p2	ycf1	2	AAATGGGGTCCAATGGCATT	ACAGGAACAAGAGGGATCCA	20	20	45.0	50.0	58.0	58.0	7.0	15.2	0.075080	137072	137191	1002-1002	1.000000	82.3
ycf1_p3	ycf1	3	AAATGGGGTCCAATGGCATT	AAGAGGGATCCACCGAAGAA	20	20	45.0	50.0	58.0	58.0	7.0	15.2	0.077666	137072	137191	994-994	1.000000	82.3
ycf1_p4	ycf1	4	AAAATGGGGTCCAATGGCAT	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.068674	137072	137191	1127-1127	1.000000	82.1
ycf1_p5	ycf1	5	AAATGGGGTCCAATGGCATT	GCAGAATACCGTCACCCATT	20	20	45.0	50.0	58.0	58.0	7.0	0.0	0.068674	137072	137191	1126-1126	1.000000	82.1
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	138597	139145	690-690	1.000000	79.1
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	138597	139145	641-641	1.000000	77.9
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.483095	138597	139145	645-645	1.000000	77.0
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	TCTCCCGTTGTTCCTCAGTA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.513605	138597	139145	694-694	1.000000	76.7
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	TTCCTCAGTAGCTCAGTGGT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.526686	138597	139145	684-684	1.000000	76.6
trnI-GAU_p1	trnI-GAU	1	TCTATTCTTTTCCCTGGCGC	AGAGAGGGATGGGGTTTCTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.151965	143984	145003	1105-1105	1.000000	79.1
trnI-GAU_p2	trnI-GAU	2	TCTATTCTTTTCCCTGGCGC	AGAGGGATGGGGTTTCTCTC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.151965	143984	145003	1103-1103	1.000000	79.1
trnI-GAU_p3	trnI-GAU	3	TCTATTCTTTTCCCTGGCGC	GAGAGGGATGGGGTTTCTCT	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.151965	143984	145003	1104-1104	1.000000	79.1
trnI-GAU_p4	trnI-GAU	4	TCTATTCTTTTCCCTGGCGC	GATGGGGTTTCTCTCGCTTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.153730	143984	145003	1098-1098	1.000000	79.1
trnI-GAU_p5	trnI-GAU	5	CGGAGCATGCATGAAGTAGT	AGAGGGATGGGGTTTCTCTC	20	20	50.0	55.0	58.1	58.1	30.6	0.0	0.168190	143984	145003	1134-1134	1.000000	79.0