Hotspot

clpP

LSC · 69587-71786 · 2200 bp

Back to Koenigia

Pi 0.0528

MarkerSeek score 78.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2200
variable_sites: 292
pi: 0.0528
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CTGAACGAGTCGCACATACA	GCAACTAGAGCCGTATGCAT	50.0	50.0	58.0	58.1	2764-2998 bp	1.0	90.8
clpP_p2	CTGAACGAGTCGCACATACA	ATACCAAGGCAAACCCATGG	50.0	50.0	58.0	58.1	2690-2924 bp	1.0	90.7
clpP_p3	CTGAACGAGTCGCACATACA	CCAGCAACTAGAGCCGTATG	50.0	55.0	58.0	58.1	2767-2961 bp	0.8	82.7
clpP_p4	CTGAACGAGTCGCACATACA	GCAAATGCCGCCATATTTGT	50.0	45.0	58.0	58.1	2626-2667 bp	0.4	58.9
clpP_p5	CTAGTACATGTTCCTCGGCG	GCAAATGCCGCCATATTTGT	55.0	45.0	57.9	58.1	2604-2645 bp	0.4	58.1