Hotspot

rpl32-trnL-UAG

SSC · 118058-118815 · 758 bp

Back to Koenigia

Pi 0.145

MarkerSeek score 78.30

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 758
variable_sites: 247
pi: 0.145
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCAGTTCCCAAAAAGCGTAC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	638-1066 bp	1.0	90.6
rpl32-trnL-UAG_p2	GCAGTTCCCAAAAAGCGTAC	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	631-1059 bp	1.0	90.1
rpl32-trnL-UAG_p3	GCAGTTCCCAAAAAGCGTAC	TGGGTAAAAATAGCACCGGG	50.0	50.0	58.0	57.9	1140-1540 bp	0.8	82.6
rpl32-trnL-UAG_p4	GCAGTTCCCAAAAAGCGTAC	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1155-1501 bp	0.4	63.5
rpl32-trnL-UAG_p5	GCAGTTCCCAAAAAGCGTAC	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	1344 bp	0.2	47.0