Hotspot

rpl33-rps18

LSC · 67335-67779 · 445 bp

Back to Koenigia

Pi 0.111

MarkerSeek score 68.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 100
pi: 0.111
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGCATACAATTCATGGCGAGA	GGCCGCTTAGATTTAAGTAAAGA	42.9	39.1	57.8	56.7	309-558 bp	1.0	52.0
rpl33-rps18_p2	AGCATACAATTCATGGCGAGA	GGCCGCTTAGATTTAAGTAAAG	42.9	40.9	57.8	55.3	309-558 bp	1.0	52.0
rpl33-rps18_p3	GCATACAATTCATGGCGAGA	GGCCGCTTAGATTTAAGTAAAGA	45.0	39.1	56.3	56.7	308-557 bp	1.0	52.0
rpl33-rps18_p4	ACAAGCATACAATTCATGGCG	GGCCGCTTAGATTTAAGTAAAGA	42.9	39.1	57.6	56.7	312-561 bp	0.9	48.0
rpl33-rps18_p5	CAAGCATACAATTCATGGCGA	GGCCGCTTAGATTTAAGTAAAGA	42.9	39.1	57.3	56.7	311-560 bp	0.9	48.0