Hotspot

psbE-petL

LSC · 64604-65224 · 621 bp

Back to Koenigia

Pi 0.0604

MarkerSeek score 82.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 621
variable_sites: 97
pi: 0.0604
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGA	55.0	45.0	58.2	56.3	682-731 bp	0.9	72.7
psbE-petL_p2	TCTCCTGTGCTTCCAGACAT	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.4	56.3	680-729 bp	0.9	71.5
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	AGCTGCTAGTAGAAAACCGAA	55.0	42.9	58.2	57.0	682-731 bp	0.9	70.8
psbE-petL_p4	TTCTCCTGTGCTTCCAGACA	AGCTGCTAGTAGAAAACCGA	50.0	45.0	58.6	56.3	681-730 bp	0.9	69.7
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	AGCTGCTAGTAGAAAACCGAA	50.0	42.9	58.4	57.0	680-729 bp	0.9	69.5