Hotspot

petA-psbJ

LSC · 63112-63821 · 710 bp

Back to Koenigia

Pi 0.0671

MarkerSeek score 79.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 119
pi: 0.0671
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCCGGACCCTGCTACTAATA	TGGGGTCAATATCCGCAATG	55.0	50.0	58.0	58.0	1518-1614 bp	1.0	91.8
petA-psbJ_p2	TTTATCCCGACGGAAGCAAG	TGGGGTCAATATCCGCAATG	50.0	50.0	58.0	58.0	1438-1534 bp	1.0	91.7
petA-psbJ_p3	CCCGGACCCTGCTACTAATA	ATGGTTGGCTATTCACGGAC	55.0	50.0	58.0	58.0	1562-1658 bp	1.0	91.6
petA-psbJ_p4	CCCGGACCCTGCTACTAATA	ACACAATCAAACCCGAACGA	55.0	45.0	58.0	58.0	1450-1546 bp	1.0	91.6
petA-psbJ_p5	CCCGGACCCTGCTACTAATA	ACGACACAATCAAACCCGAA	55.0	45.0	58.0	58.0	1453-1549 bp	1.0	91.6