Hotspot

ndhC-trnV-UAC

LSC · 50704-51596 · 893 bp

Back to Koenigia

Pi 0.09

MarkerSeek score 84.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 893
variable_sites: 190
pi: 0.09
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGCCACAAACTAGAGAGTC	GTTCAGTCCAATTGGGGTGT	55.0	50.0	58.0	58.0	1834-1911 bp	0.6	73.6
ndhC-trnV-UAC_p2	CGGCCACAAACTAGAGAGTC	GTCCAATTGGGGTGTCCATT	55.0	50.0	58.0	58.1	1829-1906 bp	0.6	73.2
ndhC-trnV-UAC_p3	TCTCACTAAAGAAGGGGCCA	GTTCAGTCCAATTGGGGTGT	50.0	50.0	58.0	58.0	1999-2076 bp	0.6	73.0
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	GTTCAGTCCAATTGGGGTGT	45.0	50.0	58.1	58.0	2023-2100 bp	0.6	72.7
ndhC-trnV-UAC_p5	CGGCCACAAACTAGAGAGTC	GCTCCCGTATCAGAATGCAA	55.0	50.0	58.0	58.0	1634 bp	0.2	47.7