Hotspot

trnT-UGU-trnL-UAA

LSC · 47012-47751 · 740 bp

Back to Koenigia

Pi 0.0968

MarkerSeek score 74.70

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 740
variable_sites: 170
pi: 0.0968
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CCCCCAGACGTCGTATTTTT	GCTTCCATTGAGTCTCTGCA	50.0	50.0	57.9	57.9	1467-1686 bp	1.0	90.3
trnT-UGU-trnL-UAA_p2	CCCCCAGACGTCGTATTTTT	GAGTCTCTGCACCTATCCCT	50.0	55.0	57.9	57.9	1666 bp	0.2	46.5
trnT-UGU-trnL-UAA_p3	TGAAGCGAAATCCCCTGAAG	GAGTCTCTGCACCTATCCCT	50.0	55.0	57.9	57.9	1548 bp	0.2	46.4
trnT-UGU-trnL-UAA_p4	GAAGCGAAATCCCCTGAAGT	GAGTCTCTGCACCTATCCCT	50.0	55.0	57.9	57.9	1547 bp	0.2	46.4
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	GAGTCTCTGCACCTATCCCT	55.0	55.0	57.9	57.9	948 bp	0.2	46.3