Hotspot

psbM-trnD-GUC

LSC · 28892-30074 · 1183 bp

Back to Koenigia

Pi 0.0708

MarkerSeek score 78.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1183
variable_sites: 205
pi: 0.0708
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ACTGTTGATTCTAGCCTGCG	GGCCCAAGAATTCGCTCATA	50.0	50.0	58.0	58.0	1773-1853 bp	0.7	79.6
psbM-trnD-GUC_p2	CTGTTGATTCTAGCCTGCGT	GGCCCAAGAATTCGCTCATA	50.0	50.0	58.0	58.0	1772-1852 bp	0.7	79.6
psbM-trnD-GUC_p3	GCAGGGCTTTTCTCAGACTT	GGCCCAAGAATTCGCTCATA	50.0	50.0	58.1	58.0	2128-2138 bp	0.4	59.6
psbM-trnD-GUC_p4	CTGTTGATTCTAGCCTGCGT	GTCTTACCGAACGCACGATA	50.0	50.0	58.0	57.8	1801-1811 bp	0.4	58.9
psbM-trnD-GUC_p5	CTGTTGATTCTAGCCTGCGT	TCTTACCGAACGCACGATAC	50.0	50.0	58.0	57.8	1800-1810 bp	0.4	58.9