Hotspot

petN-psbM

LSC · 28204-28786 · 583 bp

Back to Koenigia

Pi 0.0922

MarkerSeek score 85.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 132
pi: 0.0922
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	AAAACGGTCAGTCAAAACGA	50.0	40.0	58.2	56.1	646-707 bp	1.0	75.9
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGTCAAAACGA	50.0	40.0	57.6	56.1	585-646 bp	1.0	74.3
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AAACGGTCAGTCAAAACGAT	50.0	40.0	58.2	55.9	645-706 bp	1.0	74.1
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	AACGGTCAGTCAAAACGATT	50.0	40.0	58.2	55.9	644-705 bp	1.0	74.1
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	AAAAACGGTCAGTCAAAACGA	50.0	38.1	58.2	56.9	647-708 bp	1.0	73.9