Hotspot

rpoB-trnC-GCA

LSC · 26218-27454 · 1237 bp

Back to Koenigia

Pi 0.0743

MarkerSeek score 76.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1237
variable_sites: 229
pi: 0.0743
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	CCAGATTTGAACCGGGGAAA	50.0	50.0	58.0	58.1	1738-1919 bp	1.0	91.1
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	AATAGTGAAATCCGGGGTGC	50.0	50.0	58.0	57.9	2220-2484 bp	0.8	83.4
rpoB-trnC-GCA_p3	CGTCAAGCCCTGATCAATGA	AATAGTGAAATCCGGGGTGC	50.0	50.0	58.0	57.9	1804-2068 bp	0.8	83.4
rpoB-trnC-GCA_p4	GTCACCACCTACACAAGCAA	AATAGTGAAATCCGGGGTGC	50.0	50.0	58.0	57.9	2416-2680 bp	0.8	83.3
rpoB-trnC-GCA_p5	AAATACCGGGTCACCACCTA	AATAGTGAAATCCGGGGTGC	50.0	50.0	58.0	57.9	2425-2689 bp	0.8	83.2