Hotspot

trnS-GCU-trnG-UCC

LSC · 8084-8703 · 620 bp

Back to Koenigia

Pi 0.122

MarkerSeek score 75.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 620
variable_sites: 187
pi: 0.122
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GTTTGGCAAGCTGCTGTAAG	CATCGTTAGCTTGGAAGGCT	50.0	50.0	57.9	58.0	2024-2047 bp	0.6	74.8
trnS-GCU-trnG-UCC_p2	GTTTGGCAAGCTGCTGTAAG	CTCGCTTGCTAGATGATCCC	50.0	55.0	57.9	57.9	1822-1845 bp	0.6	74.7
trnS-GCU-trnG-UCC_p3	GTTTGGCAAGCTGCTGTAAG	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	57.9	58.1	2021-2044 bp	0.6	74.6
trnS-GCU-trnG-UCC_p4	GTTTGGCAAGCTGCTGTAAG	AGAATATGCCGCTCGATGAC	50.0	50.0	57.9	57.9	1705-1719 bp	0.4	62.2
trnS-GCU-trnG-UCC_p5	GTTTGGCAAGCTGCTGTAAG	GAATATGCCGCTCGATGACT	50.0	50.0	57.9	57.9	1704 bp	0.2	47.0