Hotspot

rps16-trnQ-UUG

LSC · 5979-6828 · 850 bp

Back to Koenigia

Pi 0.082

MarkerSeek score 75.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 168
pi: 0.082
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TGGCAGCAACATACCCTTTT	CTTACAGCAGCTTGCCAAAC	45.0	50.0	58.0	57.9	1687-1915 bp	1.0	91.5
rps16-trnQ-UUG_p2	TGGCAGCAACATACCCTTTT	TTTGGGCTGGAATTGACGAA	45.0	45.0	58.0	58.0	1329-1435 bp	0.5	71.8
rps16-trnQ-UUG_p3	TCCACACCAGATCATGTCCT	TTTGGGCTGGAATTGACGAA	50.0	45.0	58.0	58.0	966-1059 bp	0.5	71.6
rps16-trnQ-UUG_p4	TAACCCCCTTTTTGTCTGGC	TTTGGGCTGGAATTGACGAA	50.0	45.0	58.0	58.0	1600 bp	0.2	47.9
rps16-trnQ-UUG_p5	TAACCCCCTTTTTGTCTGGC	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	2065 bp	0.2	47.5