Hotspot

ndhC-trnV-UAC

LSC · 55351-56393 · 1043 bp

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Pi 0.00519
MarkerSeek score 65.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1043
variable_sites
5
pi
0.00519
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATTGGGGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1188-1330 bp 1.0 81.6
ndhC-trnV-UAC_p2 TGGTCCTTTGCTAATTGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1190-1332 bp 1.0 80.9
ndhC-trnV-UAC_p3 GTCCTTTGCTAATTGGGGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.1 57.5 1187-1329 bp 1.0 78.8
ndhC-trnV-UAC_p4 TGGTCCTTTGCTAATTGGGG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.8 57.5 1189-1331 bp 1.0 78.1
ndhC-trnV-UAC_p5 GTCCTTTGCTAATTGGGGCT GTTCGAGTCCGTATAGCCCT 50.0 55.0 58.1 58.7 1176-1318 bp 1.0 77.7