Hotspot

psbC-trnS-UGA

LSC · 38795-39041 · 247 bp

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Pi 0.0126

MarkerSeek score 63.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 3
pi: 0.0126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	388-397 bp	1.0	79.0
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	AGAACTATCGGGGGTTCGAA	45.0	50.0	58.4	58.1	345-354 bp	1.0	78.6
psbC-trnS-UGA_p3	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	422-431 bp	1.0	78.4
psbC-trnS-UGA_p4	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	387-396 bp	1.0	78.4
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	399-408 bp	1.0	78.3