Hotspot

trnD-GUC-trnY-GUA

LSC · 33152-33629 · 478 bp

Back to Koelreuteria

Pi 0.00679

MarkerSeek score 64.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 3
pi: 0.00679
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GGATTTGGATACGTCGGGAC	TGGCAATATGTCTACGCTGG	55.0	50.0	58.1	57.8	570-606 bp	1.0	81.4
trnD-GUC-trnY-GUA_p2	GGATTTGGATACGTCGGGAC	GGCAATATGTCTACGCTGGT	55.0	50.0	58.1	57.8	569-605 bp	1.0	81.4
trnD-GUC-trnY-GUA_p3	GGATTTGGATACGTCGGGAC	GGGGACGGACTGTAAATTCG	55.0	55.0	58.1	58.4	591-627 bp	1.0	80.5
trnD-GUC-trnY-GUA_p4	GGATTTGGATACGTCGGGAC	GGGACGGACTGTAAATTCGT	55.0	50.0	58.1	57.6	590-626 bp	1.0	80.3
trnD-GUC-trnY-GUA_p5	GGATTTGGATACGTCGGGAC	GATTCTTCCTGGGTCGATGC	55.0	55.0	58.1	58.4	624-660 bp	1.0	80.0