Hotspot

trnK-UUU-rps16

LSC · 4382-5394 · 1013 bp

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Pi 0.00607
MarkerSeek score 64.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1013
variable_sites
6
pi
0.00607
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CAAACTCTCCCGGTGGTATG GATAAGGGCGCTCAACCTAC 55.0 55.0 58.0 58.1 1209-1213 bp 1.0 80.8
trnK-UUU-rps16_p2 CTTACAAACTCTCCCGGTGG GATAAGGGCGCTCAACCTAC 55.0 55.0 57.9 58.1 1213-1217 bp 1.0 80.2
trnK-UUU-rps16_p3 CAAACTCTCCCGGTGGTATG CGCTCAACCTACAGGAACAG 55.0 55.0 58.0 58.3 1201-1205 bp 1.0 79.6
trnK-UUU-rps16_p4 CTTACAAACTCTCCCGGTGG CGCTCAACCTACAGGAACAG 55.0 55.0 57.9 58.3 1205-1209 bp 1.0 79.0
trnK-UUU-rps16_p5 CAAACTCTCCCGGTGGTATG AAGCAGGGGTTTTTACGGAA 55.0 45.0 58.0 57.6 1163-1167 bp 1.0 79.0