primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CAAACTCTCCCGGTGGTATG	GATAAGGGCGCTCAACCTAC	20	20	55.0	55.0	58.0	58.1	0.0	11.4	0.157009	4382	5394	1209-1213	1.000000	80.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CTTACAAACTCTCCCGGTGG	GATAAGGGCGCTCAACCTAC	20	20	55.0	55.0	57.9	58.1	0.0	11.4	0.227596	4382	5394	1213-1217	1.000000	80.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CAAACTCTCCCGGTGGTATG	CGCTCAACCTACAGGAACAG	20	20	55.0	55.0	58.0	58.3	0.0	1.3	0.309381	4382	5394	1201-1205	1.000000	79.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CTTACAAACTCTCCCGGTGG	CGCTCAACCTACAGGAACAG	20	20	55.0	55.0	57.9	58.3	0.0	1.3	0.379968	4382	5394	1205-1209	1.000000	79.0
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CAAACTCTCCCGGTGGTATG	AAGCAGGGGTTTTTACGGAA	20	20	55.0	45.0	58.0	57.6	0.0	0.0	0.392486	4382	5394	1163-1167	1.000000	79.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.241837	9191	10099	903-974	1.000000	74.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CCGACGCTTTAATCCACTCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.248863	9191	10099	903-974	1.000000	74.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	GCTCGTACAACGGATTAGCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.1	58.2	0.0	1.2	1.319982	9191	10099	925-996	1.000000	74.2
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	GCTCGTACAACGGATTAGCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.1	56.8	0.0	1.2	1.327008	9191	10099	925-996	1.000000	74.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.2	58.2	0.0	1.2	1.388603	9191	10099	961-1032	1.000000	73.6
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	TGCAAAGCGTCCATTGTCTA	AAGACATTCACCGAGGAAGC	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.277998	11158	11494	502-502	1.000000	80.6
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	AATTCGGAATGCAAAGCGTC	AAGACATTCACCGAGGAAGC	20	20	45.0	50.0	57.7	57.9	3.6	0.0	0.363177	11158	11494	511-511	1.000000	79.9
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	TGCAAAGCGTCCATTGTCTA	TCAGGAACAAATGGAACGCT	20	20	45.0	45.0	57.8	57.7	0.0	0.0	0.447746	11158	11494	458-458	1.000000	79.6
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	AATGCAAAGCGTCCATTGTC	AAGACATTCACCGAGGAAGC	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.453108	11158	11494	504-504	1.000000	79.2
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GAATGCAAAGCGTCCATTGT	AAGACATTCACCGAGGAAGC	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.453108	11158	11494	505-505	1.000000	79.2
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	GGATTTGGATACGTCGGGAC	TGGCAATATGTCTACGCTGG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.376971	33152	33629	570-606	1.000000	81.4
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	GGATTTGGATACGTCGGGAC	GGCAATATGTCTACGCTGGT	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.378523	33152	33629	569-605	1.000000	81.4
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	GGATTTGGATACGTCGGGAC	GGGGACGGACTGTAAATTCG	20	20	55.0	55.0	58.1	58.4	0.0	0.0	0.489525	33152	33629	591-627	1.000000	80.5
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	GGATTTGGATACGTCGGGAC	GGGACGGACTGTAAATTCGT	20	20	55.0	50.0	58.1	57.6	0.0	0.0	0.513202	33152	33629	590-626	1.000000	80.3
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	GGATTTGGATACGTCGGGAC	GATTCTTCCTGGGTCGATGC	20	20	55.0	55.0	58.1	58.4	0.0	0.0	0.550482	33152	33629	624-660	1.000000	80.0
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.434339	38795	39041	388-397	1.000000	79.0
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	AGCTGCAGCAGGATTTGAAA	AGAACTATCGGGGGTTCGAA	20	20	45.0	50.0	58.4	58.1	38.2	0.0	0.519035	38795	39041	345-354	1.000000	78.6
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	20	20	50.0	55.0	58.4	58.1	3.5	0.0	0.481207	38795	39041	422-431	1.000000	78.4
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	20	20	45.0	55.0	57.6	58.1	33.4	0.0	0.502562	38795	39041	387-396	1.000000	78.4
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	20	20	45.0	55.0	58.4	58.1	38.2	0.0	0.504584	38795	39041	399-408	1.000000	78.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.0	57.8	0.0	1.1	0.185568	39690	40351	816-1074	1.000000	79.6
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.374855	39690	40351	819-1077	1.000000	78.1
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.8	57.8	0.0	1.1	0.395214	39690	40351	812-1070	1.000000	77.9
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.3	57.8	0.0	1.1	0.425837	39690	40351	817-1075	1.000000	77.7
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.4	57.8	0.0	1.1	0.530110	39690	40351	815-1073	1.000000	76.9
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TCCACGGCTGAAACGTATTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.224747	52868	53651	921-1023	1.000000	79.6
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.287928	52868	53651	893-995	1.000000	79.1
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	CATCCACGGCTGAAACGTAT	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.399908	52868	53651	923-1025	1.000000	78.2
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATCCACGGCTGAAACGTATT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.448485	52868	53651	922-1024	1.000000	77.8
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TGTGTCAGTAATGGTCGGGA	TCCACGGCTGAAACGTATTT	20	20	50.0	45.0	58.4	57.8	0.0	0.0	0.537891	52868	53651	973-1075	1.000000	77.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGCTAATTGGGGCT	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.344651	55351	56393	1188-1330	1.000000	81.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGTCCTTTGCTAATTGGGG	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.427413	55351	56393	1190-1332	1.000000	80.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GTCCTTTGCTAATTGGGGCT	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.1	57.5	0.0	0.0	0.688423	55351	56393	1187-1329	1.000000	78.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTCCTTTGCTAATTGGGG	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.8	57.5	0.0	0.0	0.771185	55351	56393	1189-1331	1.000000	78.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GTCCTTTGCTAATTGGGGCT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	58.1	58.7	0.0	0.0	0.830717	55351	56393	1176-1318	1.000000	77.7
petA-psbJ_p1	petA-psbJ	1	ATCCATTACGTGTCCAAGGC	ATTCTGGATTGGGTTCGTCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.062562	68052	68922	976-1016	1.000000	83.7
petA-psbJ_p2	petA-psbJ	2	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCGTTGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.121553	68052	68922	971-1011	1.000000	83.3
petA-psbJ_p3	petA-psbJ	3	CATTACGTGTCCAAGGCCTT	ATTCTGGATTGGGTTCGTCG	20	20	50.0	50.0	58.2	58.0	13.8	0.0	0.202959	68052	68922	973-1013	1.000000	82.6
petA-psbJ_p4	petA-psbJ	4	TTTGGCATCCGTTGTTTTGG	ATTCTGGATTGGGTTCGTCG	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.255390	68052	68922	945-985	1.000000	82.2
petA-psbJ_p5	petA-psbJ	5	CATTACGTGTCCAAGGCCTT	GGATTGGGTTCGTCGTTGTA	20	20	50.0	50.0	58.2	57.9	13.8	0.0	0.261950	68052	68922	968-1008	1.000000	82.1
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.051764	69715	71154	1585-1585	1.000000	80.8
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.057305	69715	71154	1587-1587	1.000000	80.8
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.156772	69715	71154	1508-1508	1.000000	80.1
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.162314	69715	71154	1510-1510	1.000000	80.0
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	0.0	0.171450	69715	71154	1594-1594	1.000000	79.9
ycf2_p1	ycf2	1	GTGCTACAAGATCTCGTGCA	CCGCTGATGGGGTTAAATCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.055043	155688	155807	1302-1302	0.500000	59.6
ycf2_p2	ycf2	2	AGGCTTGGTATCCAGGAACT	CCGCTGATGGGGTTAAATCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.078747	155688	155807	710-710	0.500000	59.4
ycf2_p3	ycf2	3	AGATCTCGTGCATTGGAACC	CCGCTGATGGGGTTAAATCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.081173	155688	155807	1294-1294	0.500000	59.4
ycf2_p4	ycf2	4	GTGCTACAAGATCTCGTGCA	TTCTCTTTGTTTCCCGAGCC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.111499	155688	155807	1515-1515	0.500000	59.1
ycf2_p5	ycf2	5	CAGCTCCGTATCAAGGTCAC	CCGCTGATGGGGTTAAATCA	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.123439	155688	155807	854-854	0.500000	59.0