Hotspot

rpl16

LSC · 77363-78771 · 1409 bp

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Pi 0.00793

MarkerSeek score 64.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1409
variable_sites: 22
pi: 0.00793
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CAGAAAGAAAAACAGGGCGT	TCCGGAAATCCCTGGAAAAA	45.0	45.0	56.8	57.0	1557-1559 bp	1.0	64.1
rpl16_p2	CAGAAAGAAAAACAGGGCGT	TTCCGGAAATCCCTGGAAAA	45.0	45.0	56.8	57.0	1558-1560 bp	1.0	64.1
rpl16_p3	CAGAAAGAAAAACAGGGCGT	TTTCCGGAAATCCCTGGAAA	45.0	45.0	56.8	57.0	1559-1561 bp	1.0	64.1
rpl16_p4	CAGAAAGAAAAACAGGGCGT	TTTTCCGGAAATCCCTGGAA	45.0	45.0	56.8	57.0	1560-1562 bp	1.0	64.1
rpl16_p5	CAGAAAGAAAAACAGGGCGT	TTTTTCCGGAAATCCCTGGA	45.0	45.0	56.8	57.0	1561-1563 bp	1.0	64.1