Hotspot

ccsA-ndhD

SSC · 106883-107034 · 152 bp

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Pi 0.0362

MarkerSeek score 69.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 152
variable_sites: 11
pi: 0.0362
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGGCACTTGTAGCTTCGATA	AGGGTGGAGGCTCTCTTATC	50.0	55.0	57.8	57.9	304-319 bp	1.0	80.7
ccsA-ndhD_p2	CGGCACTTGTAGCTTCGATA	CAGGGTGGAGGCTCTCTTAT	50.0	55.0	57.8	58.2	305-320 bp	1.0	79.9
ccsA-ndhD_p3	CGGCACTTGTAGCTTCGATA	GGTGGAGGCTCTCTTATCCA	50.0	55.0	57.8	58.2	302-317 bp	1.0	79.9
ccsA-ndhD_p4	CGGCACTTGTAGCTTCGATA	TCTCCCTATCCGTTGACAGG	50.0	55.0	57.8	58.2	321-336 bp	1.0	79.5
ccsA-ndhD_p5	ATTCGGCACTTGTAGCTTCG	AGGGTGGAGGCTCTCTTATC	50.0	55.0	58.6	57.9	307-322 bp	1.0	77.2