Hotspot

rpl32-trnL-UAG

SSC · 105164-105780 · 617 bp

Back to Koeleria

Pi 0.0081

MarkerSeek score 49.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 617
variable_sites: 10
pi: 0.0081
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTTTTTCGGGGCAACAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.0	58.0	721 bp	1.0	81.2
rpl32-trnL-UAG_p2	TTTTTCGGGGCAACAACAAC	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.0	58.0	720 bp	1.0	81.2
rpl32-trnL-UAG_p3	TTTTTCGGGGCAACAACAAC	GGATTCGAACCGAGATGCTT	45.0	50.0	58.0	58.1	723 bp	1.0	80.8
rpl32-trnL-UAG_p4	GTTTTTCGGGGCAACAACAA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.0	58.0	1273 bp	1.0	80.5
rpl32-trnL-UAG_p5	TTTTTCGGGGCAACAACAAC	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.0	58.0	1272 bp	1.0	80.5