Hotspot

ndhF-rpl32

SSC · 104114-104974 · 861 bp

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Pi 0.00582
MarkerSeek score 63.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
861
variable_sites
10
pi
0.00582
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 945-973 bp 1.0 43.1
ndhF-rpl32_p2 AAGGCCAAATCCCATTGACA TCCAAATATTTTTACGAATACGCT 45.0 29.2 57.7 55.3 973-1001 bp 1.0 43.1
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCTT 55.0 28.0 58.1 56.0 945-973 bp 1.0 43.1
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTCCAAATATTTTTACGAATACGCT 55.0 28.0 58.1 56.0 946-974 bp 1.0 43.1
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TCTTCCAAATATTTTTACGAATACGC 55.0 30.8 58.1 56.9 948-976 bp 1.0 43.1