Hotspot

psbK-psbI

LSC · 6954-7348 · 395 bp

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Pi 0.0101

MarkerSeek score 56.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 7
pi: 0.0101
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTCCGTCCTGGGTCATTAGA	50.0	50.0	58.0	57.8	566 bp	1.0	78.9
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	50.0	50.0	57.9	57.8	517 bp	1.0	78.7
psbK-psbI_p3	CGTGGATGTTATGCCTGTCA	GAATTTTTACTCCTCGCGCC	50.0	50.0	58.0	57.5	591 bp	1.0	77.1
psbK-psbI_p4	CGTGGATGTTATGCCTGTCA	GGAATTTTTACTCCTCGCGC	50.0	50.0	58.0	57.5	592 bp	1.0	77.1
psbK-psbI_p5	CGTGGATGTTATGCCTGTCA	TCCGTCCTGGGTCATTAGAT	50.0	50.0	58.0	57.5	565 bp	1.0	77.0