Hotspot

ycf20

Genome · 148330-148713 · 384 bp

Back to Klebsormidium

Pi 0.489

MarkerSeek score 79.00

Primer available yes

Label ycf20

Feature Metadata

label_name: ycf20
feature_type: gene
region: Genome
length_bp: 384
variable_sites: 178
pi: 0.489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf20_p1	ACAGGTGACACAGGTCCATA	GCTAATCAGCCGAGCCTATC	50.0	55.0	58.0	58.0	1114 bp	0.5	59.8
ycf20_p2	ATACAGGTGACACAGGTCCA	GCTAATCAGCCGAGCCTATC	50.0	55.0	58.0	58.0	1116 bp	0.5	59.8
ycf20_p3	TACAGGTGACACAGGTCCAT	GCTAATCAGCCGAGCCTATC	50.0	55.0	58.0	58.0	1115 bp	0.5	59.8
ycf20_p4	TCCTCCGAAGAATACGACGA	GCTAATCAGCCGAGCCTATC	50.0	55.0	58.0	58.0	1397 bp	0.5	59.7
ycf20_p5	ACAGGTGACACAGGTCCATA	CGAACGGTCGTAGGTTCAAT	50.0	50.0	58.0	58.0	776 bp	0.5	59.7