Hotspot

orf441

Genome · 4878-6203 · 1326 bp

Back to Klebsormidium

Pi 0.474

MarkerSeek score 78.00

Primer available yes

Label orf441

Feature Metadata

label_name: orf441
feature_type: gene
region: Genome
length_bp: 1326
variable_sites: 467
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf441_p1	AAAAGGACACTGTGACCACC	TGTTCAGAGGCTCAGTCTCA	50.0	50.0	57.9	58.0	2288 bp	0.5	59.6
orf441_p2	AGACCATACCCGCCTCTATC	TGTTCAGAGGCTCAGTCTCA	55.0	50.0	58.1	58.0	2083 bp	0.5	59.4
orf441_p3	ACCATACCCGCCTCTATCAA	TGTTCAGAGGCTCAGTCTCA	50.0	50.0	57.9	58.0	2081 bp	0.5	59.2
orf441_p4	AAAAGGACACTGTGACCACC	TGAGCTATTATCTGCGACGC	50.0	50.0	57.9	57.9	2403 bp	0.5	59.2
orf441_p5	AATGACAGAGCGTAAACCCC	TGTTCAGAGGCTCAGTCTCA	50.0	50.0	57.9	58.0	1922 bp	0.5	59.1